AUROMEGA

AUROMEGA

Microbial production of omega-3 fatty acids – a model based approach.

Project overview

Project lead: Per Bruheim
Institution: NTNU
Partners: SINTEF
Duration: Start-up 2017

Publications

 

  • Rau, E-Ming; Aasen, Inga Marie; Bartosova, Zdenka; Bruheim, Per & Ertesvåg, Helga (2023). Utilizing lipidomics and fatty acid synthase inhibitors to explore lipid accumulation in two thraustochytrid species. Algal Research. ISSN 2211-9264. 71. doi: 10.1016/j.algal.2023.103091.
  • Eide, Marta; Goksøyr, Anders; Yadetie, Fekadu; Gilabert, Alejandra; Bartosova, Zdenka & Frøysa, Håvard Guldbrandsen [Show all 15 contributors for this article] (2023). Integrative omics-analysis of lipid metabolism regulation by peroxisome proliferator-activated receptor a and b agonists in male Atlantic cod. Frontiers in Physiology. ISSN 1664-042X. 14. doi: 10.3389/fphys.2023.1129089. Full text in Research Archive
  • Rau, E-Ming; Bartosova, Zdenka; Kristiansen, Kåre Andre; Aasen, Inga Marie; Bruheim, Per & Ertesvåg, Helga (2022). Overexpression of two new acyl-CoA:diacylglycerol acyltransferase 2-like acyl-CoA:sterol acyltransferases enhanced squalene accumulation in Aurantiochytrium limacinum. Frontiers in Microbiology. ISSN 1664-302X. 13. doi: 10.3389/fmicb.2022.822254. Full text in Research Archive
  • Rau, E-Ming & Ertesvåg, Helga (2021). Method Development Progress in Genetic Engineering of Thraustochytrids. Marine Drugs. ISSN 1660-3397. 19(9). doi: 10.3390/MD19090515. Full text in Research Archive
  • Bartosova, Zdenka; Villa Gonzalez, Susana; Voigt, Andre & Bruheim, Per (2021). High throughput semi-quantitative UHPSFC-MS/MS lipid profiling and lipid class determination. Journal of Chromatographic Science. ISSN 0021-9665. 59(7), p. 670–680. doi: 10.1093/chromsci/bmaa121.
  • Rau, E-Ming; Aasen, Inga Marie & Ertesvåg, Helga (2021). A non-canonical Δ9-desaturase synthesizing palmitoleic acid identified in the thraustochytrid Aurantiochytrium sp. T66. Applied Microbiology and Biotechnology. ISSN 0175-7598. 105, p. 5931–5941. doi: 10.1007/s00253-021-11425-5. Full text in Research Archive
  • Bartosova, Zdenka; Ertesvåg, Helga; Nyfløt, Eirin Lishaugen; Kämpe, Kristoffer; Aasen, Inga Marie & Bruheim, Per (2021). Combined Metabolome and Lipidome analyses for in-depth characterization of lipid accumulation in the DHA producing Aurantiochytrium sp. T66. Metabolites. ISSN 2218-1989. 11(3). doi: 10.3390/metabo11030135. Full text in Research Archive
  • Røst, Lisa Marie; Darestani, Armaghan Shafaei; Fuchino, Katsuya & Bruheim, Per (2020). Zwitterionic HILIC tandem mass spectrometry with isotope dilution for rapid, sensitive and robust quantification of pyridine nucleotides in biological extracts. Journal of chromatography. B. ISSN 1570-0232. 1144, p. 1–12. doi: 10.1016/j.jchromb.2020.122078. Full text in Research Archive
  • Røst, Lisa Marie; Thorfinnsdottir, Lilja Brekke; Kumar, Kanhaiya; Fuchino, Katsuya; Langørgen, Ida Eide & Bartosova, Zdenka [Show all 8 contributors for this article] (2020). Absolute Quantification of the Central Carbon Metabolome in Eight Commonly Applied Prokaryotic and Eukaryotic Model Systems. Metabolites. ISSN 2218-1989. 10(74). doi: 10.3390/metabo10020074. Full text in Research Archive
  • Voigt, Andre & Almaas, Eivind (2019). Assessment of weighted topological overlap (wTO) to improve fidelity of gene co-expression networks. BMC Bioinformatics. ISSN 1471-2105. 20(58), p. 1–11. doi: 10.1186/s12859-019-2596-9. Full text in Research Archive
  • Heggeset, Tonje Marita Bjerkan; Ertesvåg, Helga; Liu, Bin; Ellingsen, Trond Erling; Vadstein, Olav & Aasen, Inga Marie (2019). Lipid and DHA-production in Aurantiochytrium sp. – Responses to nitrogen starvation and oxygen limitation revealed by analyses of production kinetics and global transcriptomes. Scientific Reports. ISSN 2045-2322. 9, p. 1–13. doi: 10.1038/s41598-019-55902-4. Full text in Research Archive
  • Gysi, Deisy Morselli; Voigt, Andre; Fragoso, Tiago de Miranda; Almaas, Eivind & Nowick, Katja (2018). wTO: An R package for computing weighted topological overlap and a consensus network with integrated visualization tool. BMC Bioinformatics. ISSN 1471-2105. 19(392), p. 1–16. doi: 10.1186/s12859-018-2351-7. Full text in Research Archive

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  • Rau, E-Ming (2021). Utilizing inhibitors and genetic engineering to study the biosynthesis of unsaturated fatty acids and lipids in two thraustochytrid strains .
  • Rau, E-Ming (2021). A Δ12-desaturase homolog of thraustochytrid Aurantiochytrium sp. T66 has Δ9-desaturase activity and plays a role in producing palmitoleic acid and vaccenic acid .
  • Rau, E-Ming; Ertesvåg, Helga; Collier, Jackie L & Aasen, Inga Marie (2021). A Novel Protein of the Thraustochytrid Aurantiochytrium sp. T66 Homologous to Δ12-desaturases Has Δ9- desaturase Activity and Synthesizes Palmitoleic Acid.
  • Almaas, Eivind (2020). Stories of network analysis: Cellular metabolism to COVID-19.
  • Rau, E-Ming (2020). The AUROMEGA project-a brief introduction.
  • Aasen, Inga Marie; Bartosova, Zdenka; Haslene-Hox, Hanne; Heggeset, Tonje Marita Bjerkan; Stafsnes, Marit Hallvardsdotter & Bruheim, Per [Show all 7 contributors for this article] (2019). Elucidating lipid accumulation and DHA synthesis in Aurantiochytrium sp. strain T66 using -omics technologies.
  • Heggeset, Tonje Marita Bjerkan; Ertesvåg, Helga; Liu, Bin & Aasen, Inga Marie (2019). Genome annotation and transcriptome analysis of the lipid- and DHA-accumulating Aurantiochytrium sp. T66.
  • Aasen, Inga Marie (2019). Production of omega3 fatty acids for salmon feed by heterotrophic microorganisms.
  • Stafsnes, Marit Hallvardsdotter; Bartosova, Zdenka & Bruheim, Per (2019). Metabolomics and fluxomics - essential approaches in optimization of bioprocesses.
  • Bartosova, Zdenka; Gonzalez, Susana Villa & Bruheim, Per (2019). High-throughput method for lipidomic analysis and semi-quantification of lipid classes.
  • Bartosova, Zdenka; Stafsnes, Marit Hallvardsdotter; Voigt, Andre & Bruheim, Per (2019). Supercritical fluid chromatography with mass spectrometry: A versatile tool for lipid profiling and lipid class quantitation.
  • Aasen, Inga Marie; Haslene-Hox, Hanne; Ertesvåg, Helga; Heggeset, Tonje Marita Bjerkan & Bruheim, Per (2019). Fatty acid synthesis in Aurantiochytrium sp, revealed by analyses of growth and production kinetics and global proteomes. .
  • Aasen, Inga Marie; Haslene-Hox, Hanne; Stafsnes, Marit Hallvardsdotter; Heggeset, Tonje Marita Bjerkan & Bruheim, Per (2019). Lipid accumulation and DHA synthesis in Aurantiochytrium sp, elucidated by transcriptome, proteome and metabolome analyses.
  • Ertesvåg, Helga; Haslene-Hox, Hanne; Aasen, Inga Marie; Almaas, Eivind; Bartosova, Zdenka & Heggeset, Tonje Marita Bjerkan [Show all 11 contributors for this article] (2019). Industrial microbiology – 'omics methods and modelling as tools for process optimization .
  • Aasen, Inga Marie; Ertesvåg, Helga; Heggeset, Tonje Marita Bjerkan; Vadstein, Olav & Sevgeli, Huseyin (2018). Production of heterotrophic microorganisms for use as a source of long-chain omega3 fatty acids in aquafeed.
  • Aasen, Inga Marie (2018). Production of EPA/DHA by heterotrophic microorganisms: How can GMO contribute?
  • Aasen, Inga Marie; Ertesvåg, Helga; Heggeset, Tonje Marita Bjerkan; Vadstein, Olav; Broli, Gunn & Sevgeli, Huseyin [Show all 7 contributors for this article] (2018). Heterotrofe mikroorganismer som kilde for omega3-fettsyrer i fiskefôr.

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Research group

The long-chain omega-3 fatty acids EPA and DHA are essential for humans, as well as for marine fish species. The current source is fish oil. As wild fish catches cannot be further increased, continued growth of marine aquaculture, in Norway and globally, is now seriously constrained by the availability of fish oil. New, sustainable sources of the EPA and DHA are needed. Thraustochytrids are unicellular eukaryotic microorganisms able to accumulate high levels of lipids. They can be cultivated at high cell concentration and are extremely promising organisms for development of economic competitive omega-3 fatty acid bioprocesses.

Despite many years of research, there is still a lack of basic understanding of fatty acid synthesis in thraustochytrids, where DHA and saturated fatty acids are produced by two competing pathways. AUROMEGA partners NTNU and SINTEF have over the last decade isolated a high number of thraustochytrid strains and characterized their lipid-producing potential.The systems biology approach in AUROMEGA will provide an enhanced understanding of what limits the DHA synthesis in thraustochytrids and how it can be improved. An iterative approach applying high integration of experimental disciplines, with extensive omics analyses, and mathematical modelling will be used. The mathematical and computational analysis will be based on genome-scale metabolic reconstruction and simulations to predict metabolic performance profiles, and complex network analysis to identify key regulatory features of DHA-synthesis, with particular focus of increasing the rate of DHA-synthesis and introduction in the triacylglycerol storage lipids. The acquired new knowledge will be translated into enhanced DHA production capabilities of selected thraustochytrid strains and laying the foundation for a sustainable and economically feasible industrial omega-3 fatty acid production process, thereby enabling further growth of one of the most important industries in Norway.