Dataset publication: fluorescence imaging of salmon skin cells illuminated by multimodal waveguide chip
This dataset is published as the replication data for a recent publication in the journal Optics Express by Opstad et al.: Fluorescence fluctuation-based super-resolution microscopy using multimodal waveguided illumination.
Fluorescence microscopy image of actin in salmon keratocytes. The sample substrate layer is illuminated using a multimodal photonic chip. The image was generated by summing together 1500 waveguide mode pattern images.
Here, the dataset was used to demonstrate a new super-resolution imaging technique exploiting the illumination variations introduced by sequentially exciting different waveguide modes (or illumination patterns) in the sample substrate (i.e., a photonic chip). The mode patterns can be added together to achieve a high contrast image of the surface layer (known as TIRF microscopy), or computationally reconstructed for super-resolution microscopy using the method MUSICAL as demonstrated in the mentioned article. The sample was fluorescently labelled for filamentous actin in salmon keratocytes harvested from scale samples.
Opstad, Ida S., 2021, "Replication data for: Fluorescence fluctuation-based super-resolution microscopy using multimodal waveguided illumination", DataverseNO, V1