Dataset publication: fluorescence imaging of salmon skin cells illuminated by multimodal waveguide chip

This dataset is published as the replication data for a recent publication in the journal Optics Express by Opstad et al.: Fluorescence fluctuation-based super-resolution microscopy using multimodal waveguided illumination.

Fluorescence microscopy image.

Fluorescence microscopy image of actin in salmon keratocytes. The sample substrate layer is illuminated using a multimodal photonic chip. The image was generated by summing together 1500 waveguide mode pattern images.

Here, the dataset was used to demonstrate a new super-resolution imaging technique exploiting the illumination variations introduced by sequentially exciting different waveguide modes (or illumination patterns) in the sample substrate (i.e., a photonic chip). The mode patterns can be added together to achieve a high contrast image of the surface layer (known as TIRF microscopy), or computationally reconstructed for super-resolution microscopy using the method MUSICAL as demonstrated in the mentioned article. The sample was fluorescently labelled for filamentous actin in salmon keratocytes harvested from scale samples.

 

Citation:

Opstad, Ida S., 2021, "Replication data for: Fluorescence fluctuation-based super-resolution microscopy using multimodal waveguided illumination", DataverseNO, V1

Published Oct. 30, 2021 10:24 AM - Last modified Oct. 30, 2021 10:24 AM