Super-resolution images and videos of mitochondria-derived vesicles in living cardiomyoblasts

Ida S. Opstad

To sustain its steady beating, our hearts need a continuous flow of energy throughout our entire lives. The energy needed is made available by a large number of mitochondria in the heart. However, the mitochondria can during life accumulate damage that eventually leads to heart disease and even cardiac arrest. Cells have some mechanisms to ensure mitochondria stay in tip-top shape, one of these being mitochondria-derived vesicles.

Figure 1: Mitochondria-derived vesicles (MDVs) in super-resolution images were quantified with help of machine-learning based segmentation. Previously published in [1]

 As they are extremely small and delicate, their discovery is quite recent. To better understand the biology and importance of these tiny vesicles, we optimized the super-resolution imaging conditions for living heart cells and acquired fascinating videos of dynamic mitochondria and their derived vesicles. We have recently published both a quantification study of mitochondria-derived vesicles [1] and a Data Descriptor article [2] to make the data easier to reuse for other researchers. The entire dataset is made available for anyone to explore from DataverseNO https://doi.org/10.18710/PDCLAS [3].

 

References

1.         Opstad, I.S., et al., Mitochondrial dynamics and quantification of mitochondria‐derived vesicles in cardiomyoblasts using structured illumination microscopy. Journal of Biophotonics, 2021: p. e202100305.

2.         Opstad, I.S., et al., Three-dimensional structured illumination microscopy data of mitochondria and lysosomes in cardiomyoblasts under normal and galactose-adapted conditions. Scientific Data, 2022. 9(1): p. 1-7.

3.         Opstad, I.S., 3DSIM data of mitochondria in the cardiomyoblast cell-line H9c2 adapted to either glucose or galactose. 2021: DataverseNO.

 

 

Published June 13, 2022 9:22 PM - Last modified June 13, 2022 9:22 PM